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    Stratification Experiments

  • Seed Stratification Experiments [View Experiment]
  • The Effect of Cold Stratification on Germination in 28 Cultural Relict Plant Species [View Experiment]
  • Effect of seed source, light and cold-moist stratification on seed germination [View Experiment]
  • Cephalotus follicularis Seed Stratification Experiments [View Experiment]
  • Stratification Requirements for Seed Dormancy Alleviation in a Wetland Weed. [View Experiment]
  • Effects of Treatment and Seed Source on Germination of Eastern Redcedar Seed [View Experiment]
  • Propagation of Endangered Species: Variable Germination of Pink Sandverbena [View Experiment]
  • Seed & Grmination K-12 Experiments & Background Information [View Experiment]
  • Experiments on stratification of heterogeneous sand mixtures [View Experiment]
  • Stratification Background Information


    Stratification is the process of pretreating seeds to simulate natural winter conditions that a seed must endure before germination.

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    Many seed species undergo an embryonic dormancy phase, and generally will not sprout until this dormancy is broken. The time taken to stratify seeds depends on species and conditions; though in many cases two months is sufficient.

    In the wild, seed dormancy is usually overcome by the seed spending time in the ground through a winter period and having their hard seed coat softened up by frost and weathering action. By doing so the seed is undergoing a natural form of "cold stratification" or pretreatment. This cold moist period triggers the seed's embryo, its growth and subsequent expansion eventually break through the softened seed coat in its search for sun and nutrients.

    Process: For seeds of trees and shrubs from temperate climates, stratification involves soaking and chilling seeds prior to sowing. This simulates natural conditions where the seeds would remain through a winter on cold, wet ground. Seeds will usually germinate promptly and uniformly after stratification. Unstratified seeds may take up to two years to germinate, if they do so at all.

    In its most basic form, when the cold stratification process is controlled, the pretreatment amounts to nothing more than subjecting the seeds to storage in a cool (ideally +1 to +3C; not freezing) and moist environment for a period found to be sufficient for the species in question. This period of time may vary from one to three months.

    To accomplish this you merely place the seeds in a sealed plastic bag with moistened vermiculite (or sand or even a moistened paper towel) and refrigerate it. Use three times the amount of vermiculite as seeds. It is important to only slightly dampen the vermiculite, as excessive moisture can cause the seeds to grow mouldy in the bag.

    After undergoing the recommended period of cold stratification, the seeds are ready to be removed and sown in the nursery bed for germination.

    Preparing a stratifying medium: The seeds should be cleaned of any additional material (fruit pulp, leaf and seed-pod fragments, cone scales, etc), but the shells of nuts should not be removed.

    Many sources recommend using peat, a combination of peat and sand, or vermiculite as the medium for cold stratifying seeds. The medium must be sterile to prevent harm to the seed by pathogens including fungi.

    Soaking the seeds in cold water for 6-12 hours immediately before placing them in cold stratification can cut down on the amount of time needed for stratification, as the seed needs to absorb some moisture to enable the chemical changes that take place.

    Any seeds that are indicated as needing a period of warm stratification followed by cold stratification should be subjected to the same measures, but the seeds should additionally be stratified in a warm area first, followed by the cold period in a refrigerator later. Warm stratification requires temperatures of 15-20C. In many instances, warm stratification followed by cold stratification requirements can also be met by planting the seeds in summer in a mulched bed for expected germination the following spring. Some seeds may not germinate until the second spring.

    Use of fungicide: Use of a fungicide to moisten your stratifying vermiculite will help prevent fungal diseases. Chinosol (8-hydroxyquinoline sulfate), primarily a disinfectant and often recommended for growing succulents from seed prone to mold, is one such fungicide.

    Different seeds should be placed in different bags rather than putting them all into one bag, and large quantities are also best split into several small bags. That way any fungal outbreak will be restricted to only some seeds. If no fungicide is used, a close check should be kept on the seeds, removing any which show signs of mould or become soft and with a decaying smell.

    If an outbreak of fungus occurs, remove the seeds and re-apply fungicide, then place them in a new bag with new slightly moistened vermiculite. Always keep the bag sealed. The stratifying seeds should be checked on a regular basis for either fungus or germination. If any seeds germinate while in the refrigerator, they should be removed and sown.

    Sowing and seedlings: Most seedlings, whether grown in pots or beds, benefit from good air circulation which discourages fungus growth and promotes sturdy stems. Potting and germinating medium/soil is not critical as long as the soil is light as well as lightly firmed down but not heavily compacted. Sterilised potting soil will minimize problems with Botrytis or Pythium fungal disease. These problems are much more likely to occur if air circulation is poor.

    Most seeds need only be planted at a depth equal to their own thickness in order to germinate. Seeds planted outdoors are best planted little deeper to avoid disturbance caused by heavy rainfall. The soil should be slightly damp but never soaking wet, nor allowed to dry out completely.

    Seed dormancy is a condition of plant seeds that prevents germinating under optimal environmental conditions. Living, non dormant seeds, germinate when soil temperatures and moisture conditions are suited for cellular processes and division, dormant seeds do not.One important function of most seeds is delayed germination, which allows time for dispersal and prevents germination of all the seeds at same time. The staggering of germination safeguards some seeds and seedlings from suffering damage or death from short periods of bad weather or from transient herbivores; it also allows some seeds to germinate when competition from other plants for light and water might be less intense. Another form of delayed seed germination is seed quiescence, which is different than true seed dormancy and occurs when a seed fails to germinate because the external environmental conditions are too dry or warm or cold for germination. Many species of plants have seeds that delay germination for many months or years, and some seeds can remain in the soil seed bank for more than 50 years before germination. Some seeds have a very long viability period, and the oldest documented germinating seed was nearly 2000 years old based on radiocarbon dating.

    Source: Wikipedia (All text is available under the terms of the GNU Free Documentation License and Creative Commons Attribution-ShareAlike License.)

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